other:Article Title: Detection of a Point Mutation (G143A) in Cyt b of Corynespora cassiicola That Confers Pyraclostrobin Resistance
Article Snippet: LAMP Primer Design The LAMP primer was designed on the website Primer Explorer (v5) (http://primerexplorer.jp/e/ (accessed on 8 February 2021)) according to the mutation GGT (Gly) → GCT (Ala) at the 143 amino acid codon of Cyt b, and the mutation site was located at the 3′ end of the forward inner primer (FIP).
Sequencing:Article Title: LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.
Article Snippet: .. The steps for designing LAMP primers using Primer Explorer V5 are as follows: (i) launch the program, upload the correct format of the sequence file, and click on “Primer Design” to display the regions of the target sequences; (ii) click on “Generate” to get the designed primers of the best five primer options for a particular gene sequence; (iii) choose the primer set with the highest ∆G value for dimerization and click on “Display” to exhibit the designed primer list in a new window; (iv) select the primer with a ∆G value of ≤−4 kcal/mol at the 5′ end of F1c/B1c and at the 3′ end of F2/B2 and F3/B3 and click on “Confirm” to obtain the particular primer details; (v) after choosing the appropriate primer set, click on “Primer information” to save the file for loop-primer generation, and then click on “Save” to download the primer sequences in a Word/Excel document; (vi) to generate the loop primers, re-launch the software and upload the saved file from step (v); (vii) click on “Primer Design” to display the LF and LB in the target sequence; (viii) next, click on “Generate,” followed by “Display,” to obtain the designed LF and LB primers in a new window; (ix) choose the loop primer pair with the highest ∆G value for dimerization and click on “Confirm” to display the primer details; (x) finally, choose the loop primers with lowest ∆G value at 3′ end and click on “Save” to download the primers. ..
Article Title: LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection
Article Snippet: .. The steps for designing LAMP primers using Primer Explorer V5 are as follows: (i) launch the program, upload the correct format of the sequence file, and click on “Primer Design” to display the regions of the target sequences; (ii) click on “Generate” to get the designed primers of the best five primer options for a particular gene sequence; (iii) choose the primer set with the highest Δ G value for dimerization and click on “Display” to exhibit the designed primer list in a new window; (iv) select the primer with a Δ G value of ≤−4 kcal/mol at the 5′ end of F1c/B1c and at the 3′ end of F2/B2 and F3/B3 and click on “Confirm” to obtain the particular primer details; (v) after choosing the appropriate primer set, click on “Primer information” to save the file for loop-primer generation, and then click on “Save” to download the primer sequences in a Word/Excel document; (vi) to generate the loop primers, re-launch the software and upload the saved file from step (v); (vii) click on “Primer Design” to display the LF and LB in the target sequence; (viii) next, click on “Generate,” followed by “Display,” to obtain the designed LF and LB primers in a new window; (ix) choose the loop primer pair with the highest Δ G value for dimerization and click on “Confirm” to display the primer details; (x) finally, choose the loop primers with lowest Δ G value at 3′ end and click on “Save” to download the primers. ..
Software:Article Title: LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection.
Article Snippet: .. The steps for designing LAMP primers using Primer Explorer V5 are as follows: (i) launch the program, upload the correct format of the sequence file, and click on “Primer Design” to display the regions of the target sequences; (ii) click on “Generate” to get the designed primers of the best five primer options for a particular gene sequence; (iii) choose the primer set with the highest ∆G value for dimerization and click on “Display” to exhibit the designed primer list in a new window; (iv) select the primer with a ∆G value of ≤−4 kcal/mol at the 5′ end of F1c/B1c and at the 3′ end of F2/B2 and F3/B3 and click on “Confirm” to obtain the particular primer details; (v) after choosing the appropriate primer set, click on “Primer information” to save the file for loop-primer generation, and then click on “Save” to download the primer sequences in a Word/Excel document; (vi) to generate the loop primers, re-launch the software and upload the saved file from step (v); (vii) click on “Primer Design” to display the LF and LB in the target sequence; (viii) next, click on “Generate,” followed by “Display,” to obtain the designed LF and LB primers in a new window; (ix) choose the loop primer pair with the highest ∆G value for dimerization and click on “Confirm” to display the primer details; (x) finally, choose the loop primers with lowest ∆G value at 3′ end and click on “Save” to download the primers. ..
Article Title: LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection
Article Snippet: .. The steps for designing LAMP primers using Primer Explorer V5 are as follows: (i) launch the program, upload the correct format of the sequence file, and click on “Primer Design” to display the regions of the target sequences; (ii) click on “Generate” to get the designed primers of the best five primer options for a particular gene sequence; (iii) choose the primer set with the highest Δ G value for dimerization and click on “Display” to exhibit the designed primer list in a new window; (iv) select the primer with a Δ G value of ≤−4 kcal/mol at the 5′ end of F1c/B1c and at the 3′ end of F2/B2 and F3/B3 and click on “Confirm” to obtain the particular primer details; (v) after choosing the appropriate primer set, click on “Primer information” to save the file for loop-primer generation, and then click on “Save” to download the primer sequences in a Word/Excel document; (vi) to generate the loop primers, re-launch the software and upload the saved file from step (v); (vii) click on “Primer Design” to display the LF and LB in the target sequence; (viii) next, click on “Generate,” followed by “Display,” to obtain the designed LF and LB primers in a new window; (ix) choose the loop primer pair with the highest Δ G value for dimerization and click on “Confirm” to display the primer details; (x) finally, choose the loop primers with lowest Δ G value at 3′ end and click on “Save” to download the primers. ..
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